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MAK058

Sigma-Aldrich

D-Lactate Colorimetric Assay

sufficient for 100 colorimetric tests

Synonym(s):

D-Lactate Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

neurological disorders; gastrointestinal diseases

storage temp.

−20°C

General description

D-Lactate, typically present in the bloodstream at nanomolar concentrations, is produced by an intestinal source or via the methylglyoxal pathway. In mammals, D-Lactate metabolism requires D-Lactate hydrogenase and is metabolized slowly, thus an increase in blood concentration levels can lead to acidemia and acidosis. The severity of this D-lactic acidosis can be associated with neurotoxic symptoms. Significant D-Lactate accumulations in the body can also be related to impaired metabolism and excretion.

Application

D-Lactate Colorimetric Assay kit has been used to determine the stereospecificity of lactate produced.

Suitability

Suitable for use with samples of serum, plasma, cells, culture and fermentation media.

Principle

In this assay, D-Lactate is specifically oxidized by D-Lactate hydrogenase and generates a proportional colorimetric product measured at 450 nm. The useful concentration range in samples is 0.1-10 mM D-Lactate.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials


Certificates of Analysis (COA)

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Ayca Erkin-Cakmak et al.
The Journal of clinical endocrinology and metabolism, 104(7), 3003-3011 (2019-03-15)
To investigate the link between dietary sugar consumption and two separate pathogenetic mechanisms associated with metabolic syndrome: de novo lipogenesis (DNL) and nonenzymatic glycation. We assessed changes in serum d-lactate (the detoxification end-product of methylglyoxal) concentration in response to 9
William H. Hoffman et al.
Journal of clinical research in pediatric endocrinology, 12(2), 160-167 (2019-09-14)
To determine the time relationships of soluble receptor for glycation end-products (sRAGE), [a decoy of the advanced glycation end-products (AGE)-RAGE axis] and D-lactate, (a metabolite of methylglyoxal) in the inflammatory response to diabetic ketoacidosis (DKA). Sixteen children and adolescents with
Yanan Chen et al.
Journal of animal science and biotechnology, 12(1), 70-70 (2021-06-11)
Intestinal disorder is an important factor contributing to growth lag and high rates of morbidity and mortality of piglets with intrauterine growth retardation (IUGR). Resveratrol (RSV) and its derivative pterostilbene (PT) are natural stilbenes possessing various bioactivities, such as antioxidative
Poonam Naik et al.
Current eye research, 45(2), 184-189 (2019-08-31)
Purpose: Microbiological investigations of vitreous fluid have often failed to detect the causative agent in infectious endophthalmitis resulting in a clinical dilemma. D-Lactate is a byproduct of bacterial metabolism, and its accumulation in sterile body fluids indicates bacterial infection. The
Reyna Rodríguez-Mortera et al.
Clinical chemistry and laboratory medicine, 56(7), 1100-1108 (2018-02-16)
Childhood obesity is associated with insulin resistance (IR), increased levels of small dense low-density lipoprotein (sd-LDL) as well as with augmented hepatic de novo lipogenesis, which implies increased triose phosphate fluxes that may lead to increased methylglyoxal (MG) and its

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