The compound should be dissolved initially at 50mg/mL in a 2:1 mixture of Formic acid and water, respectively. Once dissolved, the stock can be diluted in a buffer or culture medium. This is not a sterile product. Stock solutions should be filter sterilized prior to adding to sterile medium.
H9636
Hypoxanthine
powder, suitable for cell culture, BioReagent
Synonym(s):
6-Hydroxypurine
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All Photos(4)
About This Item
Empirical Formula (Hill Notation):
C5H4N4O
CAS Number:
Molecular Weight:
136.11
Beilstein:
5811
EC Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75
Pricing and availability is not currently available.
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Product Name
Hypoxanthine, powder, BioReagent, suitable for cell culture
product line
BioReagent
Quality Level
Assay
≥99%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
>300 °C (lit.)
solubility
formic acid: water (2:1): 50 mg/mL
SMILES string
O=C1NC=Nc2nc[nH]c12
InChI
1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)
InChI key
FDGQSTZJBFJUBT-UHFFFAOYSA-N
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General description
Hypoxanthine (6-hydroxypurine), a purine derivative is a naturally occurring compound. It is the deaminated form of adenine and a breakdown product of adenosine monophosphate (AMP).[1]
Application
Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.
Hypoxanthine has been used:
- as a supplement in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) -buffered Roswell park memorial institute (RPMI) to culture Plasmodium falciparum strain IT/FCR3[2]
- in the preparation of Dulbecco′s modified eagle′s medium (DMEM) + MXH for the selection of chimeric modified vaccinia virus ankara (MVA)[3]
- to generate superoxide anions[4]
- to prepare hybridoma selection and maintenance media.
Biochem/physiol Actions
Hypoxanthine is capable of stimulating cell death. It can also induce reactive oxygen species (ROS). It results in endothelial dysfunction via apoptosis, stimulated by oxidative stress.[5]
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Binding of Plasmodium falciparum to CD36 can be shielded by the glycocalyx
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Malaria Journal, 16(1), 1-11 (2017)
Hypoxanthine causes endothelial dysfunction through oxidative stress-induced apoptosis
Kim YJ, et al.
Biochemical and biophysical research communications, 482(4), 821-827 (2017)
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PLoS neglected tropical diseases, 10(3), e0004481-e0004481 (2016-03-11)
The ability of Paracoccidioides to defend itself against reactive oxygen species (ROS) produced by host effector cells is a prerequisite to survive. To counteract these radicals, Paracoccidioides expresses, among different antioxidant enzymes, superoxide dismutases (SODs). In this study, we identified
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What is the solubility of HYPOXANTHINE in water and How to dissolve HYPOXANTHINE in water for cell culture.
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