G4913
Anti-Glutamic Acid Decarboxylase 65 (514-530) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-GAD 65
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse, pig, human, rat
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using tissue sections of rat pancreas
microarray: suitable
western blot: 1:4,000 using rat brain extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)
General description
GAD is predominantly expressed in the central nervous system (CNS) and pancreatic islets. It is also expressed in testis, oviduct and ovary. GAD exists in two isofoms, GAD 65 and GAD 67. GAD65 is an ampiphilic, membrane-anchored protein, (585 amino acid residues) and is encoded on human chromosome 10.
Specificity
Reacts specifically with GAD 65 (65 kDa) from brain.
Immunogen
synthetic peptide corresponding to the C-terminal region of human GAD 65 (amino acids 514-530). The sequence is identical in mouse GAD 65 and highly conserved in rat and pig GAD 65 but not found in GAD 67.
Application
Anti-Glutamic Acid Decarboxylase 65 antibody produced in rabbit is suitable as a primary antibody for the determination of expression of GAD 65 isoform in putative P boutons in rat and mouse at a working dilution of 1:5000 and in lysates prepared from rat testis at different developmental ages and rat cerebellum by immunoblotting.
It is also suitable for immunohistochemistry at a working dilution of 1:500 using formalin-fixed, paraffin-embedded sections of rat pancreas and for microarray.
It is also suitable for immunohistochemistry at a working dilution of 1:500 using formalin-fixed, paraffin-embedded sections of rat pancreas and for microarray.
Biochem/physiol Actions
Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to γ-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. It has been identified as an autoantigen in insulin-dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS) and may serve as a marker in the early stages of IDDM. GAD 65 synthesizes transmitter GABA for vesicular release.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
D I Hughes et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(25), 9038-9043 (2005-06-11)
Presynaptic inhibition of primary muscle spindle (group Ia) afferent terminals in motor nuclei of the spinal cord plays an important role in regulating motor output and is produced by a population of GABAergic axon terminals known as P boutons. Despite
N J Tillakaratne et al.
Journal of neurochemistry, 58(2), 618-627 (1992-02-01)
gamma-Aminobutyric acid (GABA) and its synthetic enzyme, glutamate decarboxylase (GAD), are not limited to the nervous system but are also found in nonneural tissues. The mammalian brain contains at least two forms of GAD (GAD67 and GAD65), which differ from
M G Erlander et al.
Neurochemical research, 16(3), 215-226 (1991-03-01)
Studies of the GABA-synthetic enzyme glutamate decarboxylase (glutamic acid decarboxylase; GAD; E.C.4.1.1.15) began in 1951 with the work of Roberts and his colleagues. Since then, many investigators have demonstrated the structural and functional heterogeneity of brain GAD. At least part
Haixiong Liu et al.
Acta biochimica et biophysica Sinica, 41(7), 545-553 (2009-07-07)
Glutamate decarboxylase 65 (GAD65) produces gamma-aminobutyric acid, the main inhibitory neurotransmitter in adult mammalian brain. Previous experiments, performed in brain, showed that GAD65 gene possesses two TATA-less promoters, although the significance is unknown. Here, by rapid amplification of cDNA ends
M Solimena et al.
Trends in neurosciences, 14(10), 452-457 (1991-10-01)
Stiff-Man syndrome (SMS) is a disorder of the CNS, characterized by rigidity of the body musculature, which has been hypothesized to result from an impairment of GABAergic neurotransmission. GABA is the main inhibitory neurotransmitter of the brain. It is also
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service