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C4482

Sigma-Aldrich

CelLytic™ Y Cell Lysis Reagent

For yeast cells

Synonym(s):

Yeast cell lysis reagent

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About This Item

UNSPSC Code:
41116134
NACRES:
NA.56
Pricing and availability is not currently available.

Quality Level

200

form

solution

General description

CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.

Application

CelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein,[1] heat shock protein (ssa1)[2] and human interleukin 4 (hIL4).[3]

Features and Benefits

  • Fast: Cell lysis in just 20 minutes
  • Efficient: Three times more efficient than glass beads
  • Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
  • Gentle: Non-denaturing - preserves biological activity

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000373971
0000373970
0000342070
0000342932
0000327291

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DNA damage induced nucleotide excision repair in Saccharomyces cerevisiae
Singh RK and Krishna M
Molecular and Cellular Biochemistry, 290(1-2), 103-112 (2006)
Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity
Li R, et al.
Protein Expression and Purification, 96, 1-7 (2014)
The beta6beta7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation
Xu L, et al.
Cellular and Molecular Life Sciences, 75(8), 1445-1459 (2018)

Questions

1–7 of 7 Questions  

  1. Does this contain EDTA or other chelators?

    1 answer

    1. This product does not contain EDTA or other chelators.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. What strains have been used with CelLytic™ Y Cell Lysis Reagent, Product C4482?

    1 answer

    1. The efficiency of CelLytic Y reagent for protein extraction has been tested on different strains of Saccharomyces cerevisiae [Y187, Y190, W303(a), S288C, SP1, S1278b (diploid and haploid), BJ2168, and cdc25] and Schizosaccharomyces pombe.

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  4. Can I use CelLytic™ Y Cell Lysis Reagent, Product C4482, to make spheroplasts?

    1 answer

    1. No.  For spheroplast formation and protein extraction we recommend the CelLytic Y Plus kit.

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  5. Should I use CelLytic™ Y Cell Lysis Reagent, Product C4482?

    1 answer

    1. For all cases in which the addition of DTT does not interfere with the application tested, it is recommended to add DTT (5 - 10 mM final concentration) to the CelLytic Y reagent.  Addition of DTT significantly improves total protein yield.

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  6. What is in CelLytic™ Y Cell Lysis Reagent, Product C4482?

    1 answer

    1. The formulation is considered proprietary, but we can state that it is a non-denaturing phosphate-free reagent.

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  7. Can I use glass beads with CelLytic™ Y Cell Lysis Reagent, Product C4482?

    1 answer

    1. If cell growth conditions are not optimal, then glass beads can be used to increase yields.

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