72464
Atto 565 NHS ester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
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About This Item
Empirical Formula (Hill Notation):
C35H34ClN3O11
Molecular Weight:
708.11
MDL number:
UNSPSC Code:
12352108
NACRES:
NA.32
Recommended Products
product line
BioReagent
Assay
≥90% (HPLC)
≥90% (degree of coupling)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
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General description
Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.
Features and Benefits
- Strong absorption
- High fluorescence quantum yield
- High thermal and photo-stability
Other Notes
Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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