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A92775

Sigma-Aldrich

Arsenazo III

calcium-sensitive dye, Powder

Synonym(s):

2,2′-(1,8-Dihydroxy-3,6-disulfonaphthylene-2,7-bisazo)bisbenzenearsonic acid, 2,7-Bis(2-arsonophenylazo)chromotropic acid

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About This Item

Linear Formula:
(HO)2C10H2(SO3H)2(N=NC6H4AsO3H2)2
CAS Number:
Molecular Weight:
776.37
Beilstein:
5717957
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Arsenazo III, calcium-sensitive dye

Quality Level

form

powder

technique(s)

titration: suitable

impurities

<0.1% Ca

color

brown to very dark brown, and Black

mp

>320 °C (lit.)

solubility

ammonium hydroxide: 1 mg/mL

λmax

530 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(\N=N\c2ccccc2[As](O)(O)=O)c(cc3cc(c(\N=N\c4ccccc4[As](O)(O)=O)c(O)c13)S(O)(=O)=O)S(O)(=O)=O

InChI

1S/C22H18As2N4O14S2/c29-21-18-11(9-16(43(37,38)39)19(21)27-25-14-7-3-1-5-12(14)23(31,32)33)10-17(44(40,41)42)20(22(18)30)28-26-15-8-4-2-6-13(15)24(34,35)36/h1-10,29-30H,(H2,31,32,33)(H2,34,35,36)(H,37,38,39)(H,40,41,42)/b27-25+,28-26+

InChI key

UQHVTNUJRKELCE-NBHCHVEOSA-N

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Application

Arsenazo III has been used as a color forming reagent to determine uranium concentration.

Biochem/physiol Actions

Arsenazo III is a metallochromic dye. Arsenazo III is used in the determination of calcium in biological samples. It is used to evaluate calcium transport in permeabilized cells. It is also used for the detection of rare earth metals (polyvalent metal ions). Arsenazo III is used for the spectrophotometric determination of uranium and thorium.

Pictograms

Skull and crossbonesEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Achraf Ammar et al.
Nutrition & metabolism, 17, 31-31 (2020-04-24)
Pomegranate juice (POMj) contains abundant soluble polyphenolic antioxidant compounds and is recommended for its cardioprotective/atheroprotective properties. However, very few studies have investigated the efficacy of POMj supplementation to alter physiological responses during intensive physical exercise. This placebo-controlled study aimed to
Kenichiro Sato et al.
Journal of Alzheimer's disease : JAD, 68(2), 777-788 (2019-03-01)
Effect of serum calcium level to the incidence of mild cognitive impairment (MCI) conversion to early Alzheimer's disease (AD) remains uncertain. To investigate association between baseline serum calcium and the MCI conversion in the Japanese Alzheimer's Disease Neuroimaging Initiative (J-ADNI)
Muhammad Haleem Khan et al.
Chemosphere, 63(7), 1165-1169 (2005-11-22)
A short, sensitive and reliable spectrophotometric method, which has advantages over all known "wet chemistry" methods for uranium determination with regard to tolerance to common interferences, has been developed for the determination of uranium. Selectivity, molar absorptivity and the determination
Changes in the intracellular concentration of free calcium ions in a pace-maker neurone, measured with the metallochromic indicator dye arsenazo III.
Gorman AL and Thomas MV
The Journal of Physiology, 275, 357-376 (1978)
Purification of arsenazo III, a Ca2+-sensitive dye.
Kendrick NC
Analytical Biochemistry, 76.2, 487-501 (1976)

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