UFC5050

Amicon^® Ultra Centrifugal Filter, 50 kDa MWCO

sample volume 0.5 mL, regenerated cellulose membrane, MWCO 50 kDa

• Synonym(s): Centrifugal concentrator, 50 kDa MWCO, 0.5 mL sample volume
• UNSPSC Code: 41104916
• eCl@ss: 36100101
• NACRES: NB.24

Properties

• material: polypropylene cap; polypropylene tube; regenerated cellulose membrane; styrene-butadiene housing
• Quality Level: 400
• sterility: non-sterile
• product line: Amicon^®
• manufacturer/tradename: Amicon^® Ultra
• parameter: 0.5 mL sample volume
• technique(s): protein extraction: suitable; protein purification: suitable
• L: 4.99 cm (1.96 in.)
• diam.: 10.8 mm
• filtration area: 1 cm²
• volume: 0.5 mL
• pore size: 50 kDa MWCO
• shipped in: ambient

Description

General description

Amicon^® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, BSA (67 kDa) at 1 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon^® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon^® Ultra 0.5 mL filters deliver great performance and lower spin times.

Application

• Ultrafiltration with 50,000 Dalton molecular weight cutoff Ultracel^® membrane
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
• Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
• Desalting, buffer exchange, and protein dialysis

Features and Benefits

• Ultracel^® regenerated cellulose membrane with 50 kDa NMWCO delivers retentate recovery of greater than 90%
• Concentrates 500 μL down to 15 μL
• Concentration factor 25x to 30x
• High sample recovery (greater than 90% of dilute starting solution)
• Fast processing time: 10 to 30 minutes
• Reverse spin capability provides consistent recoveries

Linkage

Replaces: 42415

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany