17-295
Chromatin Immunoprecipitation (ChIP) Assay Kit
Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.
Synonym(s):
Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.41
Recommended Products
Quality Level
manufacturer/tradename
EZ-ChIP
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
General description
For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.
Packaging
Kit capacity: 22 Assays
Components
Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)
All necessary buffers
All necessary buffers
Quality
Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.
Physical form
Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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