Skip to Content
Merck
All Photos(2)

Documents

06-1102

Sigma-Aldrich

Anti-Protein patched homolog 1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

PTCH protein +12b, PTCH protein +4′, PTCH protein -10, patched (Drosophila) homolog, patched homolog (Drosophila), patched homolog 1 (Drosophila)

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PTCH1(5727)

General description

Protein patched homolog 1 is a receptor for sonic hedgehog, a member of the hedgehog family of cell signaling proteins. Hedgehog signaling is regulated by Protein patched homolog 1 which keeps the pathway turned off in the absences of activation. This inhibits the function of associated downstream network components such as Smoothened (Smo).

Specificity

This antibody recognizes the cytoplasmic domain of Protein patched homolog 1.

Immunogen

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Protein patched homolog 1.

Application

Anti-Protein patched homolog 1 Antibody detects level of Protein patched homolog 1 & has been published & validated for use in WB, IH.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Protein patched homolog 1 in kidney tissue.
Research Category
Signaling

Quality

Evaluated by Western Blot in human skeletal muscle, fetal tissue lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Protein patched homolog 1 on 10 µg of human skeletal muscle, fetal tissue lysate.

Target description

~ 160 kDa

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human skeletal muscle, fetal tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Chao Tang et al.
Cell regeneration (London, England), 12(1), 3-3 (2023-02-01)
The overlapping roles of Hippo and Hedgehog signaling in biological functions and diseases prompt us to investigate their potential interactions. Activation of Hippo signaling enhances the transcriptional output of Hedgehog signaling, and the role of Hippo signaling in regulating Hedgehog
Jean Marie Delalande et al.
Frontiers in molecular neuroscience, 14, 757646-757646 (2022-01-11)
TALPID3/KIAA0586 is an evolutionary conserved protein, which plays an essential role in protein trafficking. Its role during gastrointestinal (GI) and enteric nervous system (ENS) development has not been studied previously. Here, we analyzed chicken, mouse and human embryonic GI tissues
Claudia M Kowolik et al.
Journal of experimental & clinical cancer research : CR, 38(1), 431-431 (2019-10-30)
Pancreatic cancer is one of the most lethal malignancies due to frequent late diagnosis, aggressive tumor growth and metastasis formation. Continuously raising incidence rates of pancreatic cancer and a lack of significant improvement in survival rates over the past 30 years
Chengyun Xu et al.
Mucosal immunology, 11(5), 1306-1315 (2018-06-06)
Sonic hedgehog (SHH) is abundantly expressed and critical for morphogenesis in embryonic lungs; however, SHH expression drops to a much lower level in mice from E17.5 and in humans from the 21st gestational week. We find that SHH expression is
A Almazán-Moga et al.
British journal of cancer, 117(9), 1314-1325 (2017-09-08)
Rhabdomyosarcoma (RMS) is the most common type of soft tissue sarcoma in children. The Hedgehog (HH) pathway is known to develop an oncogenic role in RMS. However, the molecular mechanism that drives activation of the pathway in RMS is not

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service