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T0950

Sigma-Aldrich

Anti-γ-Tubulin (QG-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

Drosophila

technique(s)

western blot: 0.2-0.4 μg/mL using whole cell extract of the Schneider′s Drosophila Line 2 [D.mel.(2), SL2]

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

γ-Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells.
γ-tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Specificity

The sequence is highly specific for Drosophila γ-tubulin and is not found in γ-tubulin of other species or other members of the tubulin family such as α-, β-, δ-, and ε-tubulins.

Immunogen

synthetic peptide corresponding to the C-terminus of Drosophila γ-tubulin (amino acids 441-457 with N-terminal added cysteine-glycine), conjugated to KLH.

Application

Anti-γ-Tubulin (QG-17) antibody has been used in immunoblotting and immunostaining.

Biochem/physiol Actions

γ-tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michelle Kowanda et al.
Biology open, 5(8), 1040-1051 (2016-07-17)
The centrosome-associated proteins Ninein (Nin) and Ninein-like protein (Nlp) play significant roles in microtubule stability, nucleation and anchoring at the centrosome in mammalian cells. Here, we investigate Blastoderm specific gene 25D (Bsg25D), which encodes the only Drosophila protein that is
Overexpression of γ-tubulin in non-small cell lung cancer.
Maounis NF, et al.
Histology and Histopathology, 27, 1183-1194 (2012)
The gamma-tubulin gene family in humans.
Wise DO, et al.
Genomics, 67, 164-170 (2000)
Causes and consequences of gray matter heterotopia.
Watrin F, et al.
CNS Neuroscience & Therapeutics, 21, 112-122 (2015)
Human TUBG2 gene is expressed as two splice variant mRNA and involved in cell growth.
Ohashi T, et al.
Febs Letters, 590, 1053-1063 (2016)

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