SRE0113

Bst Max DNA Polymerase

recombinant, expressed in E. coli, Salt and Inhibitor Tolerant

• Synonym(s): Bst DNA polymerase, Isothermal DNA Polymerase
• UNSPSC Code: 12352200
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• specific activity: 40,000 U/mg
• feature: Salt and Inhibitor Tolerant
• optimum pH: 8.5
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Loop-mediated isothermal amplification (LAMP) has emerged as a key alternative to polymerase chain reaction (PCR)-based techniques in the amplification of nucleic acids. While conventional PCR employs a thermal cycler for repetitive cycling temperatures when amplifying, isothermal amplification techniques such as LAMP occur at a single and fixed temperature allowing the use of simple, portable, and more robust instruments for fast and exponential amplification.
The Bst Max DNA polymerase is an in-silicon designed homolog of Bst DNA Polymerase (large fragment) suitable for DNA amplification at elevated temperatures with an optimum of 65°C. Bst Max DNA polymerase is a salt-tolerant recombinant polymerase (optimal salt concentration 50-350 mM) with more than two-fold enhanced strand-displacement activity and processivity when compared to Bst polymerase. The Bst enzyme is active from 25 to 65°C. It lacks 5’-3’- and 3’-5’-exonuclease activity.

Application

Bst Max DNA Polymerase is

• Ideal for isothermal applications such as LAMP and RT-LAMP for its superior amplification performance and robustness, especially at point-of-care diagnostics
• Compatible with a wide range of buffer formulations and isothermal applications such as Ramification amplification (RAM), NEMA (Nicking enyzme-mediated amplification), HAD (Helicase-dependent amplification), MDA/SDA (Multiple/Strand displacement amplification), RCA (Rolling circle amplification), RPA (Recombinase Polymerase amplification)

Features and Benefits

• Efficient strand displacement activity allows the polymerase to amplify at a faster rate while displacing the second DNA strand during synthesis.
• High processivity ensures that the polymerase can amplify more nucleotides consecutively without dissociation from the DNA template
• Higher salt and inhibitor tolerance ensures enhanced amplification performance without the need for sample prep steps
• Ideal for amplification of most sample types, especially small and impure samples
• Triton-free formulation complies with the European Chemicals Agency’s REACH regulations to improve the protection of human health and the environment from risks that can be posed by chemicals

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Reconstitution

In solution.

Other Notes

Order primers for LAMP using our online configurator. For assistance with primer design prior to purchase, please use the online design tool from our partner.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable