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Key Documents

BCK-FC488-50

Sigma-Aldrich

EdU Flow Cytometry Kit 488

sufficient for 50 assays

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.22

fluorescence

λex 496 nm; λem 516 nm

shipped in

wet ice

storage temp.

2-8°C

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General description

The Baseclick EdU Flow Cytometry Kits provide a superior alternative to BrdU and [3H]thymidine assays for directly measuring DNA synthesis. EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU Flow Cytometry Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU Flow Cytometry Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.

Application

Flow Cytometry

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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