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01494

Sigma-Aldrich

Nancy-520

greener alternative

suitable for RT-PCR, ≥95.0% (HPCE)

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About This Item

EC Number:
200-664-3
UNSPSC Code:
12171500
NACRES:
NA.32

Quality Level

100

Assay

≥95.0% (HPCE)

form

liquid

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

solubility

DMSO: soluble

fluorescence

λex 520 nm; λem 554 nm in TE buffer; DNA

greener alternative category

Aligned,

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry

Application

Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520

Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520

Greener alternative to Ethidium bromide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup - Solvent

Flash Point(C)

87 °C - closed cup - Solvent

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nolan, T. and Bustin, S.A.
PCR Technology: Current Innovations (2013)
Park, D.J.
PCR Protocols (2011)
Rodriguez-Lazaro, D.
Real-Time PCR in Food Science: Current Technology and Applications (2013)
Saunders, N.A. and Lee, M.A.
Real-Time PCR: Advanced Technologies and Applications (2013)
van Pelt-Verkuil, E., van Belkum, A. and Hays, J.P
Principles and Technical Aspects of PCR Amplification (2008)
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Articles

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Related Content

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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