This product is a mixture of human serum albumin (HSA) and human gamma-globulin, the latter of which is essentially all human IgG. The E1% (at 280 nm) values for HSA and human IgG are 14 and 5.3, respectively. The lot-specific extinction coefficient for the mixture can be calculated using the protein concentrations provided the certificate of analysis (CofA)._x000D_
Sample calculation, for Lot SLCN8399:_x000D_
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P8119-BULKSLCN8399.pdf_x000D_
The CofA reports an HSA concentration of 4.9 g/dL, and (by difference) a gamma-globulin concentration of 3.3 g/dL. The extinction coefficient at 280 nm for this 8.2 g/dL (82 mg/mL) lot is 86. Here is the calculation: (4.9 X 14) + (3.3 X 5.3) = 86.09, which would round to 86. If the standard were diluted by a factor of 8.2 to give a 1 g/dL or 1% solution, the E1% would be 10.5.
P8119
Protein Standard
analytical standard, 80 mg/mL (HSA and gamma-globulins)
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About This Item
UNSPSC Code:
85151701
NACRES:
NA.24
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biological source
human
Quality Level
grade
analytical standard
form
liquid
contains
0.05% sodium azide as preservative
analyte chemical class(es)
amino acids, peptides, proteins
concentration
80 mg/mL (HSA and gamma-globulins)
technique(s)
gel permeation chromatography (GPC): suitable
application(s)
food and beverages
format
multi-component solution
storage temp.
2-8°C
Application
Protein standard has been used in the determination of protein content of cod trypsin isoenzymes using bio-rad protein assay and ultraviolet-visible light spectrophotometry (UV-Vis).[1]
Other Notes
Human serum albumin and gamma-globulins
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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What is the extinction coefficient for this 80 mg/mL protein standard?
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