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31659

Supelco

o-Tolidine

analytical standard

Synonym(s):

3,3′-Dimethylbenzidine, 4,4′-Bianisidine

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About This Item

Linear Formula:
[-C6H3(CH3)-4-NH2]2
CAS Number:
Molecular Weight:
212.29
Beilstein:
2210640
EC Number:
MDL number:
UNSPSC Code:
41116107
PubChem Substance ID:
NACRES:
NA.24

grade

analytical standard

Quality Level

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

128-131 °C (lit.)

application(s)

cleaning products
cosmetics
food and beverages
personal care

format

neat

SMILES string

Cc1cc(ccc1N)-c2ccc(N)c(C)c2

InChI

1S/C14H16N2/c1-9-7-11(3-5-13(9)15)12-4-6-14(16)10(2)8-12/h3-8H,15-16H2,1-2H3

InChI key

NUIURNJTPRWVAP-UHFFFAOYSA-N

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General description

o-Tolidine is widely used in the aniline dye industry for the synthesis of different azo dyes.

Application

o-Tolidine (3,3′-dimethylbenzidine) may be used as an analytical reference standard for the determination of the analyte formed from azo colorants in toy products by high-performance liquid chromatography with UV detection.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Sensitive colorimetric reagent for gold and for free chlorine in water.

Warning

May be carcinogenic.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

212.0 °F - closed cup

Flash Point(C)

100 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Carcinogenic properties of orthotolidine (3, 3?-dimethylbenzidine).
Pliss GB and Zabezhinsky MA
Journal of the National Cancer Institute, 45(2), 283- 295 (1970)
Determination of aromatic amines formed from azo colorants in toy products.
Garrigos MC
Journal of Chromatography A, 976(1-2), 309-317 (2002)
Shinpei Kawarai et al.
The Journal of veterinary medical science, 72(2), 131-140 (2009-11-27)
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Human & experimental toxicology, 18(9), 552-559 (1999-10-19)
Azo dyes represent the major class of dyestuffs. They are metabolised to the corresponding amines by liver enzymes and the intestinal microflora following incorporation by both experimental animals and humans. For safety evaluation of the dermal exposure of consumers to
Zhijun Cao et al.
Biosensors & bioelectronics, 23(3), 348-354 (2007-06-06)
The electrooxidation of o-tolidine (oTD) was investigated via the electrochemical quartz crystal microbalance (EQCM) technique. The formation and breakage of the poorly soluble charge-transfer complex (CTC) occurred during the redox switching of oTD, and the CTC precipitation on and its

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