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PC05L

Sigma-Aldrich

Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

lyophilized, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

does not contain

preservative

species reactivity

rat, human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... FOS(2353)

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~50-62 kDa c-Fos protein.
Recognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.
The proto-oncogene fos has been implicated in cell growth, differentiation and development. fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
This Anti-c-Fos (Ab-2) (4-17) Rabbit pAb is validated for use in Frozen Sections, WB, IF, IP, Paraffin Sections, Gel Shift, for the detection of c-Fos (Ab-2) (4-17).

Immunogen

Human
a synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos

Application

Frozen Sections (5-10 µg/ml)

Immunoblotting (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg per reaction)

Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)

Gel Shift (see comments and application references)

Free-floating Sections (not recommended)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from PBS, a volatile buffer, 100 µg BSA.

Reconstitution

Resuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.

Analysis Note

Positive Control
Hep G2 or NIH 3T3 cells

Other Notes

DeTogni, P., et al. 1988. Cell Biol.8, 2251.
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell51, 513.
Muller, R. 1986. Biochim. Biophys. Acta823, 207.
Verma, I. 1986. Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433
For gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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