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MABS27

Sigma-Aldrich

Anti-Diglycyl Lysine (Clone GX41) Antibody

clone GX41, from mouse

Synonym(s):

Diglycyl Lysine

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GX41, monoclonal

species reactivity

human, mouse, rat

species reactivity (predicted by homology)

all

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

shipped in

wet ice

target post-translational modification

unmodified

General description

Anti-Diglycyl Lysine has been shown to specifically detect peptides derived from the ubiquitinated portion of proteins. Diglycyl Lysine, clone GX41 is thought to find diglycyl-lysine moieties that are created and left behind at places of ubiquitination after digestion with trypsin has occurred.

Specificity

Antibody recognizes lysine residues modified by diglycine, a residue left at sites of ubiquitination after trypsin digestions.
Broad species cross-reactivity expected based on 100% sequence homology.

Immunogen

Linear peptide corresponding to Diglycyl Lysine.

Application

Anti-Diglycyl Lysine (Clone GX41) Antibody detects level of Diglycyl Lysine (Clone GX41) & has been published & validated for use in WB & IP.
Immunoprecipitation Analysis: 10 µg of Anti-Diglycyl Lysine immunoprecipitated peptides containing Diglycyl Lysine modification from isolated ubiquitinated proteins which were obtained from a human cell line.

Mass Spectrometry Analysis: A previous lot was used by an independent a laboratory in Mass Spec. Image courtesy of Samie Jaffrey, Cornell Medical College.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Quality

Evaluated by Western Blot in BSA protein and Diglycine modified BSA.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Diglycyl Lysine on 10 µg of BSA protein and Diglycine modified BSA.

Target description

Molecular weight can vary depending on the size of the protein or peptides containing Diglycyl modified lysine residues.

Physical form

Format: Purified
Protein G
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
BSA protein and Diglycine modified BSA

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Meghan C Burke et al.
Journal of proteome research, 13(12), 5965-5972 (2014-10-07)
We provide evidence at the molecular level that ubiquitinated proteins are present in exosomes shed by myeloid-derived suppressor cells (MDSC). Ubiquitin was selected as a post-translational modification of interest because it is known to play a determinant role in the
Niladri K Sinha et al.
eLife, 9 (2020-08-04)
Translation of aberrant mRNAs induces ribosomal collisions, thereby triggering pathways for mRNA and nascent peptide degradation and ribosomal rescue. Here we use sucrose gradient fractionation combined with quantitative proteomics to systematically identify proteins associated with collided ribosomes. This approach identified

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