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Key Documents

1.06022

Sigma-Aldrich

Sodium dodecyl sulfate

for biochemistry 10% in H₂O

Synonym(s):

Dodecyl sulfate sodium salt, SDS, Lauryl sulfate sodium salt, Dodecyl sodium sulfate

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About This Item

UNSPSC Code:
12352107
NACRES:
NC.01

Quality Level

form

liquid

pH

9 (20 °C in H2O)

density

1.012 g/cm3 at 20 °C

storage temp.

15-25°C

General description

Dodecyl sulfate sodium or sodium dodecyl sulfate (SDS) is an ionic detergent comprising a polar sulfate head group and a hydrophobic 12-carbon chain.

Biochem/physiol Actions

Dodecyl sulfate sodium or sodium dodecyl sulfate (SDS) combines effectively with the positively charged and hydrophobic residues of proteins using sulfate groups and alkyl chains. This detergent is very valuable in the field of structural biology and protein folding/unfolding studies. SDS can initiate the generation of α-helices in a protein structure. It stimulates cooperative unfolding in proteins like bovine serum albumin (BSA), β-lactoglobulin, α-lactalbumin, and hen egg-white lysozyme structures at elevated concentrations.

Analysis Note

Assay (two-phase titration): 9.8 - 10.2 %

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Protocols

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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