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Sigma-Aldrich

Anti-acetyl-Histone H4 Antibody

1 mg/mL, Upstate®

Synonym(s):

H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

Tetrahymena sp., human

manufacturer/tradename

Upstate®

concentration

1 mg/mL

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

acetylation (Lys5,Lys8,Lys 12,Lys16)

Gene Information

human ... H4C1(8359)

Related Categories

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificity

Other species not tested.
Recognizes acetylated histone H4.
Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May crossreact with other acetylated proteins.

Immunogen

Epitope: a.a. 2-19
KLH-conjugated peptide corresponding to amino acids 2-19 of Tetrahymena histone H4 (AGG(Ac)KGG(Ac)KGMG(Ac)KVGA(Ac)KRHS-C), acetylated on lysines 5,8,12 and 16.

Application

Anti-acetyl-Histone H4 Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a & has been validated in ChIP, ICC, IP & WB.
Immunoprecipitation:
5 μg of a previous lot immunoprecipitated acetylated histone H4 from 500 μg of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours.

Immunocytochemistry:
5-10 μg/mL of a previous lot lot gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1% paraformaldehyde in PBS.

Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate acetylated histone H4 from chromatin.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Routinely evaluated by western blot on acid-extracted proteins from HeLa cells treated with sodium butyrate.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate. A previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.

Target description

10 kDa

Physical form

Format: Purified
Protein A chromatography
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.

Storage and Stability

1 year at -20°C from date of shipment.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
TSA-treated NIH/3T3, A431, L929 (mouse) and C6 (rat) cell extracts, human breast carcinoma.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The role of protein arginine methylation in the formation of silent chromatin.
Yu, MC; Lamming, DW; Eskin, JA; Sinclair, DA; Silver, PA
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Hassana Fathallah et al.
Blood cells, molecules & diseases, 40(2), 166-173 (2007-11-22)
K562 erythroleukemia cells have been widely used as a model for the study of globin gene regulation. A number of agents have been shown to activate or suppress globin gene expression in these cells. However, the molecular effects of these
The binding of the ubiquitous transcription factor Sp1 at the locus control region represses the expression of beta-like globin genes.
Feng, D; Kan, YW
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Role of epigenetic modifications in normal globin gene regulation and butyrate-mediated induction of fetal hemoglobin.
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