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G005664

LC/MS Analysis of Amino Acids on Ascentis® Express HILIC

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] 13 mM ammonium acetate in 10:90 (v/v) water: acetonitrile; [B] 13 mM ammonium acetate in water
gradient: held at 0% B for 1 min; 0 to 90% B in 19 min
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ESI(+)
injection: 10 μL
sample: 10 mg/L in Mobile Phase A

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Tryptamine
    Tyramine
    Tryptophan
    α-Methyl-DL-methionine
    β-Alanine

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