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SAB4200372

Sigma-Aldrich

Anti-WASH1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-FAM39E, Anti-WAS protein family homolog 1, Anti-WASH

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~72 kDa

species reactivity

human, rat, mouse

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of rat NRK cells.
western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing mouse WASH1.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog) is a new member of the WASP family. Similar to other WASP family members, it contains a C-terminal for ′WH2, connecting and acidic′ (WCA) domain that binds to Actin related protein 2/actin related protein 3 (Arp2/3). In addition, WASH1 also contains a short proline-rich region, a unique N-terminal domain termed WASH-homology domain (WAHD1), and a tubulin-binding region.

Immunogen

synthetic peptide corresponding to an internal region of human WASH1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Application

Anti-WASH1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Biochem/physiol Actions

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), is a nucleation-promoting factor at the surface of endosomes. It recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of endosomes. WASH1 forms part of a multiprotein complex composed of FAM21, KIAA1033 strumpellin and WASH-interacting protein (SWIP) and coiled coil domain containing 53 (CCDC53). It associates with tubulin and localizes to early and recycling endosomes, where together with the Arp2/3 complex and actin, it is required for maintaining the shape of the endosomal compartment and the regulation of the retrograde transport.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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DNAJC13 p. Asn855Ser, implicated in familial parkinsonism, alters membrane dynamics of sorting nexin 1
Follett J, et al.
Neuroscience Letters, 706(5), 114-122 (2019)
Missense variant in CCDC22 causes X-linked recessive intellectual disability with features of Ritscher-Schinzel/3C syndrome
Kolanczyk M, et al.
European Journal of Human Genetics, 23(5), 633-633 (2015)
WASH and the Arp2/3 complex regulate endosome shape and trafficking
Duleh SN and Welch MaD
Cytoskeleton (Hoboken, N.J.), 67(3), 193-206 (2010)
A FAM21-containing WASH complex regulates retromer-dependent sorting
Gomez TS and Billadeau DD
Developmental Cell, 17(5), 699-711 (2009)
Mateusz Kolanczyk et al.
European journal of human genetics : EJHG, 23(5), 633-638 (2014-06-12)
Ritscher-Schinzel syndrome (RSS)/3C (cranio-cerebro-cardiac) syndrome (OMIM#220210) is a rare and clinically heterogeneous developmental disorder characterized by intellectual disability, cerebellar brain malformations, congenital heart defects, and craniofacial abnormalities. A recent study of a Canadian cohort identified homozygous sequence variants in the

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