Skip to Content
Merck
All Photos(2)

Documents

SAB4200273

Sigma-Aldrich

Anti-Orai1 antibody, Mouse monoclonal

clone ORAI1-89, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-CRACM1, Monoclonal Anti-ORAI calcium release-activated calcium modulator 1, Monoclonal Anti-ORAT1, Monoclonal Anti-TMEM142A, Monoclonal Anti-calcium release-activated calcium channel protein 1, Monoclonal Anti-transmembrane protein 142A

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

ORAI1-89, monoclonal

form

buffered aqueous solution

mol wt

antigen ~30 kDa (non-glycosylated Orai1)
antigen ~50 kDa (glycosylated Orai1)

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human Orai1.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ORAI1(84876)

General description

Monoclonal Anti-Orai1 (mouse IgG1 isotype) is derived from the hybridoma ORAI1-89 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide located near the C-terminus of human Orai1, conjugated to KLH. Orai1, also called Calcium release-activated calcium channel protein 1 (CRACM1), is an evolutionary conserved plasma membrane protein. Orai1 is predicted to contain four transmembrane domains with its N- and C-termini in the cell cytoplasm.
ORAI calcium release-activated calcium modulator 1 (Orai1), also known as transmembrane protein 142A (TMEM142A), is encoded by the gene mapped to human chromosome 12q24.31.{49}. The encoded protein contains four transmembrane helices with highly conserved glutamate residues in transmembrane helices 1 and 3.

Immunogen

synthetic peptide located near the C-terminus of human Orai1, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse.

Application

Monoclonal Anti-Orai1 antibody has been used in
  • immunoblotting,
  • immunofluorescence
  • immunoprecipitation
  • proximity ligation assay.

Monoclonal Anti-Orai1 antibody produced in mouse has been used in proximity ligation assay.

Biochem/physiol Actions

Orai1 essential for store-operated calcium entry in T cells and fibroblasts. Store-operated calcium entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels, following Ca2+ depletion from the endoplasmic reticulum (ER) stores, caused by stimulation of immune cells. This process is crucial for gene transcription, proliferation and cytokine release. Mutations in two highly conserved glutamate residues, E106 and E190, diminish Ca2+ influx and promote changes in ion selectivity, providing strong evidence that Orai is a pore subunit of the CRAC channel. Orai1 co-localizes with STIM1 (stromal interaction molecule 1) near the plasma membrane after store depletion. A point mutation in Orai1 is responsible for the genetic defect in store-operated Ca2+ entry and I-CRAC in cells of the hereditary severe combined immune deficiency (SCID) syndrome patients.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Orai1 and STIM Reconstitute Store-operated Calcium Channel Function
Soboloff J
The Journal of Biological Chemistry, 281, 20661-20665 (2006)
The Orai1 severe combined immune deficiency mutation and calcium release-activated Ca2+ channel function in the heterozygous condition
Thompson J L, et al.
The Journal of Biological Chemistry, 284(11), 6620-6626 (2009)
Control of type I interferon-induced cell death by Orai1-mediated calcium entry in T cells
Yue C, et al.
The Journal of Biological Chemistry, 287(5), 3207-3216 (2012)
Orai1 is an essential pore subunit of the CRAC channel
Prakriya M
Nature, 443, 230-233 (2006)
Dynamic interaction of SARAF with STIM1 and Orai1 to modulate store-operated calcium entry.
Albarran L
Scientific Reports (2016)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service