N7161

Nogo-66(1-40) antagonist peptide

≥84% (HPLC)

• Synonym(s): NEP (1-40)
• Empirical Formula (Hill Notation): C₂₀₄H₃₂₂N₅₆O₆₄ · xC₂HF₃O₂
• Molecular Weight: 4583.08 (free base basis)
• MDL number: MFCD06411437
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• Quality Level: 100
• Assay: ≥84% (HPLC)
• form: lyophilized solid
• color: white
• solubility: H₂O: 1 mg/mL
• UniProt accession no.: Q9NQC3
• storage temp.: −20°C
• Gene Information: human ... RTN4(57142)

Description

Amino Acid Sequence

Arg-Ile-Tyr-Lys-Gly-Val-Ile-Gln-Ala-Ile-Gln-Lys-Ser-Asp-Glu-Gly-His-Pro-Phe-Arg-Ala-Tyr-Leu-Glu-Ser-Glu-Val-Ala-Ile-Ser-Glu-Glu-Leu-Val-Gln-Lys-Tyr-Ser-Asn-Ser-NH2

Application

Nogo-66(1-40) antagonist peptide has been used as a Nogo-66 receptor antagonist peptide:

• to study the preliminary therapeutic effect after inhibition of Nogo-A in the cauda equina compression (CEC) model
• to determine the effects of Nogo-A/NgR1 on autophagic activation
• to study its role in Nogo-B mediated axonal branching using Schwann cells and sensory neurons of mice

Biochem/physiol Actions

Myelin-derived axon outgrowth inhibitors, such as Nogo, may account for the lack of axonal regeneration in the central nervous system (CNS) after trauma in adult mammals. Nogo-66 can inhibit axonal outgrowth through an axonal Nogo-66 receptor (NgR). Competitive antagonists of NgR derived from amino-terminal peptide fragments of Nogo-66. The Nogo-66(1 40) antagonist peptide (NEP1 40) blocks Nogo-66 or CNS myelin inhibition of axonal outgrowth in vitro, demonstrating that NgR mediates a significant portion of axonal outgrowth inhibition by myelin. Intrathecal administration of NEP1 40 to rats with mid-thoracic spinal cord hemisection results in significant axon growth of the corticospinal tract, and improves functional recovery. Thus, Nogo-66 and NgR have central roles in limiting axonal regeneration after CNS injury, and NEP1-40 provides a potential therapeutic agent.

Legal Information

Sold under a non-exclusive license. For research use only, and not for diagnostic or therapeutic use or for use in humans.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves