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H9786

Sigma-Aldrich

Anti-Hepatocyte Growth Factor Receptor (c-Met) antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-HGF Receptor

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

technique(s)

flow cytometry: suitable
immunohistochemistry: 5-15 μg/mL
western blot: 0.1-0.2 μg/mL

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MET(4233)
mouse ... Met(17295)

General description

The antibody neutralizes receptor-ligand interaction. At 0.5-2 μg/mL the antibody will block 50% of the binding of 5 ng/mL recombinant human HGF to 0.1 μg immobilized recombinant human HGF R/c-MET Fc chimera. >90% of the binding will be blocked by 10 μg/mL of the antibody.

Immunogen

purified recombinant human hepatocyte growth factor receptor extracellular domain expressed in mouse NSO cells.

Application

Anti-Hepatocyte Growth Factor Receptor (c-Met) antibody produced in goat was used to block c-Met in HeLa cells.

Biochem/physiol Actions

Hepatocyte Growth Factor (HGF) is a multifunctional, heparin-binding factor that stimulates the proliferation of liver cells. The cellular effects of HGF are mediated by its receptor, c-Met, that leads to significant activation of ERK, Src/FAK, STAT3 and PI3K signaling. Various functions of the HGF/c-Met signaling include proliferation, cell survival, motility and adhesion and tissue regeneration. Apart from liver, HGF also mediates regeneration of kidney, lung and gastro-intestines.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Simone Buraschi et al.
The Journal of biological chemistry, 285(53), 42075-42085 (2010-10-27)
A theme emerging during the past few years is that members of the small leucine-rich proteoglycan gene family affect cell growth by interacting with multiple receptor tyrosine kinases (RTKs), mostly by a physical down-regulation of the receptors, thereby depriving tumor
Hedwig S Kruitwagen et al.
Liver international : official journal of the International Association for the Study of the Liver, 31(7), 940-949 (2011-07-08)
Although the liver has a large regenerative capacity, in many hepatopathies, these repair mechanisms fail. The therapeutic potential of hepatocyte growth factor (HGF) has been proven in numerous toxin-induced liver failure models in rodents, but never in spontaneously occurring liver
Jesus Pérez-Hernández et al.
Life (Basel, Switzerland), 11(9) (2021-09-29)
Amoebiasis in humans is caused by the protozoan parasite Entamoeba histolytica, which cytotoxic activity has been demonstrated on a wide variety of target cells. The process involves the adherence of the parasite to the cell, and such adherence is mediated
H Ohmichi et al.
The American journal of physiology, 270(6 Pt 1), L1031-L1039 (1996-06-01)
Hepatocyte growth factor (HGF) has mitogenic, morphogenic, and motogenic activities on epithelial cells and plays important roles in regeneration of the liver and the kidney. We previously found that the expression of HGF gene is rapidly induced in the lung
Toshikazu Nakamura et al.
Proceedings of the Japan Academy. Series B, Physical and biological sciences, 86(6), 588-610 (2010-06-17)
It has been more than 25 years since HGF was discovered as a mitogen of hepatocytes. HGF is produced by stromal cells, and stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis in various organs via tyrosine phosphorylation of its receptor

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