FA20
Fructose Assay Kit
sufficient for 20 assays
Synonym(s):
Fructose Test Kit
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About This Item
Recommended Products
usage
sufficient for 20 assays
analyte chemical class(es)
sugars (fructose)
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Application
This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.
Kit Components Only
Product No.
Description
- fructose standard 500 mg
- glucose assay reagent 1 mL/vial
- phosphoglucose isomerase 1 mL/vial
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
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Official Methods of Analysis of the AOAC, 28-28 (1995)
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