A4550

Agar

Type A, suitable for plant cell culture

• Synonym(s): Agar-agar, Gum agar
• Linear Formula: (C₁₂H₁₈O₉)_n
• CAS Number: 9002-18-0
• EC Number: 232-658-1
• MDL number: MFCD00081288
• UNSPSC Code: 50405901
• eCl@ss: 42040102
• NACRES: NA.21

Properties

• biological source: algae (Rhodophyceae)
• type: Type A
• form: powder
• technique(s): cell culture | plant: suitable
• transition temp: transition temp 32-38 °C (1.5%)
• gel strength: >650 g/cm²
• application(s): agriculture
• storage temp.: room temp
• InChI: 1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
• InChI key: GYYDPBCUIJTIBM-DYOGSRDZSA-N

Description

General description

Agar (Type A) is extracted from agarocytes of the red alga Rhodophyceae. It is a polysaccharide complex, consisting of a neutral gelling fraction, agarose, and a non-gelling fraction, agaropectin. Type-A agar forms a thermoreversible gel, and is most suitable for plant cell culture.

Application

Type A agar has been used:

• to perform metal(loid)-related phenotyping of the Arabidopsis thaliana metal-sensitive mutant strains
• as a component of Murashige and Skoog (MS) media to culturing Arabidopsis thaliana seeds
• to culture Cicer arietinum L. (Chickpea) seeds for Agrobacterium rhizogenes mediated root transformation

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves