Skip to Content
Merck
All Photos(1)

Documents

97875

Sigma-Aldrich

Atto 647

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD05865408
UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

Assay

≥90% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
647 nm

fluorescence

λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 645-651 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 647 is a zwitterionic dye with a net electrical charge of zero. It is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH. Atto 647 is a new label with high molecular absorption (120,000) and quantum yield (0.20) as well as enough Stokes shift between excitation and emission maximum. Atto 647 is characterized by a high photostability and brightness. In addition, this label has good water solubility and low triplet formation ability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 4

1 of 4

Atto 647N maleimide BioReagent, suitable for fluorescence, ≥90% (HPLC)

Supelco

05316

Atto 647N maleimide

Atto 590 BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sigma-Aldrich

70425

Atto 590

Atto 700 BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

Sigma-Aldrich

30674

Atto 700

Sigma-Aldrich

Sigma-Aldrich

95349

Atto 647N amine

Cytokinesis,Volume 137 of Methods in Cell Biology (2017)
Jean-Manuel Segura et al.
The Journal of biological chemistry, 283(35), 24254-24263 (2008-06-27)
CD8(+) cytotoxic T lymphocytes (CTL) can recognize and kill target cells expressing only a few cognate major histocompatibility complex (MHC) I-peptide complexes. This high sensitivity requires efficient scanning of a vast number of highly diverse MHC I-peptide complexes by the
Duncan Hill et al.
Biomedical microdevices, 13(4), 759-767 (2011-05-12)
This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a
Laura Pastorino et al.
Journal of colloid and interface science, 357(1), 56-62 (2011-02-26)
The protein collagen is the major component of connective tissue and it is involved in many biological functions. Its degradation is at the basis of different pathological processes. The up-regulated expression of matrix metalloproteinases and the down-regulated expression of their
Dagmar Hildebrand et al.
Infection and immunity, 79(1), 220-228 (2010-10-20)
Pasteurella multocida is a Gram-negative bacillus that infects a number of wild and domestic animals, causing respiratory diseases. Toxigenic Pasteurella multocida strains produce a protein toxin (PMT) that leads to atrophic rhinitis in swine due to enhanced osteoclastogenesis and the
View All Related Papers

Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service