G006163
HPLC Analysis of Exenatide Acetate on Proteomix® SCX-NP5
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Proteomix SCX NP5, 25 cm x 4.6mm I.D., 5 μm particles (Z777161)
mobile phase: (A) 10 mM monosodium phosphate:acetonitrile (56:44, v/v), adjusted to pH to 2.0 with phosphoric acid; ((B) 0.5 M NaCl/10 mM monosodium phosphate:acetonitrile (56:44, v/v), adjusted to pH to 2.0 with phosphoric acid)
gradient: 0 to 65% B in 35 min; held for 5 min; to 0% B in 10 min
flow rate: 1.0 mL/min
pressure: 1653 psi (114 bar)
column temp.: 40 °C
detector: UV, 235 nm
injection: 20 μL
sample: 0.325 mg/mL exenatide acetate in mobile phase A
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
Legal Information
analytical column
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