ZMS1184

Anti-APN/CD13 Antibody, clone BR2 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): AP-N, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, CD13, EC:3.4.11.2, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, hAPN
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone BR2 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 109.5 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable
• isotype: IgG1κ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_001141.2
• UniProt accession no.: P15144
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... ANPEP(290)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone BR2 is a ZooMAb^® Mouse recombinant monoclonal antibody that specifically detects aminopeptidase N/CD13. It targets an epitope within the extracellular domain.

Immunogen

Human foreskin fibroblasts

Application

Quality Control Testing

Evaluated by Immunocytochemistry in PC3 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected APN/CD13 in PC3 cells.

Tested Applications

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected C-Myc/DDK-tagged recombinant Human Aminopeptidase N/CD13.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected APN/CD13 in one million Human peripheral blood mononuclear cells (PBMC).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Aminopeptidase N (UniProt: P15144; also known as EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13) is encoded by the ANPEP (also known as APN, CD13, PEPN) gene (Gene ID: 290) in human. Aminopeptidase N is a Zn2+-dependent, single-pass type II membrane protein that is also found as a soluble form in cells. It consists of a short N-terminal cytoplasmic end (aa 2-8), a transmembrane domain (aa 9-32), and a large extracellular portion (aa 33-967), which is composed of a Ser/Thr-rich region (aa 33-68) and the metalloprotease domain (aa 69-967). It is widely expressed as a homodimer of 280 kDa on the cell surface in many tissues, including intestinal epithelia and the nervous system. It serves as a broad specificity aminopeptidase that plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It preferentially cleaves N-terminus neutral amino acids, most notably alanine residue. Aminopeptidase N is involved in many physiological processes, including antigen presentation regulation, differentiation, proliferation, apoptosis, cancer metastasis, and angiogenesis. It is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Aminopeptidase N is associated with the growth of different human cancers and is considered to be a suitable target for anti‐cancerous therapy. Its levels are reported to be upregulated in response to hypoxia, angiogenic growth factors, and signals regulating capillary tube formation during angiogenesis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, L., et al. (2012). Proc. Natl. Acad. Sci. USA. 109 (44); 17966-17971; Wickstrom, M., et al. (2011). Cancer Sci. 102(3): 501-508; Noren, K., et al. (1997). Exp. Cell Res. 231(1); 112-118).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable