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TP0300

Sigma-Aldrich

Total Protein Kit, Micro Lowry, Peterson′s Modification

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1 KIT
NOK 2,910.00

NOK 2,910.00

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1 KIT
NOK 2,910.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

NOK 2,910.00

Please contact Customer Service for Availability

usage

sufficient for ~200 assays

Quality Level

200

General description

This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.

Application

Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.[1][2][3]

Features and Benefits

  • Complete kit for protein assays
  • Most widely cited protein assay
  • Less protein to protein variation than other assays
  • Contains reagents that can remove interfering substances

Principle

An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.

Signal Word

Danger

Hazard Statements

H290,H302,H314,H335,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000391120
0000391118
0000391120
0000391118
SLCR2114

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Expression of alpha-gustducin and alpha-transducin, G proteins coupled with taste receptors, in boar sperm
Spinaci M, et al.
Theriogenology, 82(1), 144-151 (2014)
Alberto Elmi et al.
Animals : an open access journal from MDPI, 10(3) (2020-03-12)
The roe deer (Capreolus capreolus) represents a spontaneous model of testicular inactivation: During winter, bucks show a suspension of spermatogenesis that starts again in spring and peaks during the breeding season (July-August). The underlying mechanisms to the regulation of the
Carlotta Lambertini et al.
Frontiers in veterinary science, 7, 158-158 (2020-04-17)
Proteinase activated receptor 4 (PAR4) in the gastrointestinal tract is involved in the regulation of inflammation and pain pathways. The aim of the present study was to evaluate the distribution and expression of PAR4 in the jejunum of healthy horses
Assay of proteins in the presence of interfering materials.
A Bensadoun et al.
Analytical biochemistry, 70(1), 241-250 (1976-01-01)
Paul Kaufmann et al.
PloS one, 14(8), e0220866-e0220866 (2019-08-08)
Dipeptidyl amino-peptidase 3 (DPP3) is an aminopeptidase involved in peptide degradation, including hormone peptides as angiotensin II and enkephalins. DPP3 plasma activity increases in septic patients and correlates with mortality risk. However, the exact physiological role of DPP3 remains unclear
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Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

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Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

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