SRP3049

GDF-2 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Synonym(s): BMP-9, Growth/Differentiation Factor-2
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in CHO cells
• Assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 0.5-1.9 ng/mL
• mol wt: 24.1 kDa
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: Q9UK05
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... GDF2(2658)

Description

General description

GDF-2 belongs to the TGF-β cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. GDF-2 is expressed mainly in nonparenchymal cells of the liver, but is also found in other various cells and tissues. GDF-2 can signal through the ALK1 receptor and has been implicated in a number of physiologic events. These include regulation of the hepatic reticuloendothelial system, glucose homeostasis, and iron homeostasis, as well as the inhibition of angiogenesis. Recombinant human GDF-2 is a 24.1 kDa disulfide linked homodimeric protein consisting of two 110 amino acid polypeptide chains.

Biochem/physiol Actions

GDF-2 belongs to the TGF-β cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. Recombinant human GDF-2 is a 24.1 kDa disulfide linked homodimeric protein consisting of two 110 amino acid polypeptide chains.

Sequence

SAGAGSHCQK TSLRVNFEDI GWDSWIIAPKE YEAYECKGGC FFPLADDVTPTK HAIVQTLVHL KFPTKVGKAC CVPTKLSPIS VLYKDDMGVP TLKYHYEGMS VAECGCR

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable