SAB4200075

Anti-VAC14 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-ArPIKfyve, Anti-TAX1-binding protein 2, Anti-TAX1BP2, Anti-TAX1BP2 TAX-reactive protein x, Anti-TRX
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~82 kDa
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μL
• concentration: ~1.5 mg/mL
• technique(s): immunoprecipitation (IP): 10-15 μg using A431 cell lysates; indirect immunofluorescence: 8-16 μg/mL using HeLa cells; western blot: 1-2 μg/mL using extracts of mouse brain (S1 fraction) or rat brain (S1 fraction)
• UniProt accession no.: Q08AM6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VAC14(55697) ; rat ... Vac14(307842)

Description

General description

VAC14 gene is mapped to human chromosome 16q22.1-q22.2. VAC14 is a scaffold protein and is a component of the PIKfyve protein kinase complex.

Specificity

Anti-VAC14 (C-terminal) specifically recognizes human, mouse, and rat VAC14.

Application

Anti-VAC14 (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Mutation in VAC14 gene leads to the development of retinitis pigmentosa (RP).

VAC14 is a regulator of PIP5K3/PIKfyve, a dual specificity kinase.

VAC14 phosphorylates PtdIns(3)P to generate the housekeeping phospholipid PtdIns(3,5)P₂. It associates with and upregulate PIKfyve phosphoinositide-5-kinase activity. VAC14-PIP5K3- PtdIns(3,5)P₂ pathway is thought to be physiologically linked to insulin activation of glucose transport into the cell. Knockout of the VAC14 gene in mice results in massive neurodegeneration and particularly affected are neurons in the midbrain and of the peripheral sensory system.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable