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SAB4200041

Sigma-Aldrich

Anti-Talin antibody,Mouse monoclonal

clone TA205, purified from hybridoma cell culture

Synonym(s):

Anti-TLN

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

TA205, monoclonal

form

buffered aqueous solution

mol wt

antigen ~225 kDa

species reactivity

chicken, bovine, human

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 0.5-1 μg/mL using HeLa cells
western blot: 0.5-1.0 μg/mL using HeLa/Jurkat cell extracts

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

chicken ... Tln1(395194)
human ... TLN1(7094)

General description

Monoclonal Anti-Talin (mouse IgG1 isotype) is derived from the hybridoma TA205 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with partially purified preparation of human platelet talin. Talin is present in areas of cell-substratum and cell-cell contacts. Two types of talin were found in human, talin 1 and talin 2. Talin 1 co-distributes with integrins in the cell surface membrane. The C-terminus comprises binding sites for proteins such as β-1-integrin, actin, and vinculin. The N-terminus of the protein consists of elements for localization to cell extracellular matrix junctions. Talin 2 has a different pattern of expression compared to talin 1, but talin1 associates with unique transmembrane receptors to form novel linkages between extracellular matrices and actin cytoskeleton.
Talin 1 (TLN1) gene mapped to human chromosome 9p13.3, codes for a 270kDa cytoskeletal protein containing 2541 amino acids. Talin-1 is widely expressed in the kidney, liver, spleen, stomach, lung and vascular smooth muscle. The TLN1 antiparallel dimeric structure is characterized with a globular head containing four-point-one, ezrin, radixin, moesin (FERM) domain and rod domain involved in dimer formation.

Immunogen

partially purified preparation of human platelet talin

Application

Monoclonal Anti-Talin antibody produced in mouse has been used in western blotting and immunostaining.
Monoclonal Anti-Talin antibody produced in mouse has been used in:
  • Immunoprecipitation
  • immunofluorescence
  • immunocytochemistry

Biochem/physiol Actions

Talin 1 (TLN1) is a cytoskeletal protein, which plays a vital role in cancer cell adhesion, migration and invasion. It is also involved in integrin activation and it facilitates the adhesion of integrin to actin cytoskeleton. TLN1 might act as a potential prognostic biomarker in nasopharyngeal carcinoma (NPC). Overexpression of TLN1 is observed in endometrioid carcinoma, oral squamous cell carcinoma (OSCC), prostate cancer and hepatocellular carcinoma. Serum TLN1 functions as a potential biomarker of hepatocellular carcinoma (HCC). Talin 1 (TLN1) is highly expressed in glioblastoma multiforme (GBM). The inhibition of TLN1 activity helps in diminishing malignant features of GBM cells. It also plays an important role in regulation of, Bevacizumab (anti-angiogenic agent) -resistance.
Talin 1 assists in the attachment of adherent cells to the extracellular matrix and of lymphocytes to other cells. It plays an important role in the assembly of actin filaments and in spreading and migration of different cell types, such as fibroblasts and osteoclasts.

Target description

Talin is a cytoskeletal protein that is found concentratedin areas of cell-substratum and cell-cell contacts. Theencoded protein plays a significant role in the assemblyof actin filaments and in spreading and migration ofvarious cell types, includ

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gene duplication and functional divergence during evolution of the cytoskeletal linker protein talin
Senetar MA and McCann RO
Gene, 362, 141-152 (2005)
D R Critchley
Biochemical Society transactions, 32(Pt 5), 831-836 (2004-10-21)
The cytoskeletal proteins talin and vinculin form part of a macromolecular complex on the cytoplasmic face of integrin-mediated cellular junctions with the extracellular matrix. Recent genetic, biochemical and structural data show that talin is essential for the assembly of such
YAP regulates cell mechanics by controlling focal adhesion assembly
Nardone G
Nature Communications (2017)
High expression of Talin-1 is associated with poor prognosis in patients with nasopharyngeal carcinoma.
Xu YF
BMC Cancer (2015)
Ming-Tsung Lai et al.
The Journal of pathology, 224(3), 367-376 (2011-05-07)
Oral squamous cell carcinoma (OSCC) is highly invasive and is associated with frequent tumour recurrences and lymph node metastases. Identification of genes involved in the aggressiveness of OSCC may provide new targets for clinical intervention. A genome-wide study based on

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