The suitability of Rhodamine 123 for staining mitochondria is not specifically tested during quality control testing. However, it is expected that Rhodamine 123 can be used for this purpose. Conn's Biological Stains, 10th Edition does state that Rhodamine 123 can be used for staining mitochondria. Reference: Celis JS (1998) Cell Biology: A Laboratory Handbook, 2nd Edition v1, Academic Press, San Diego.
R8004
Rhodamine 123
Powder
Synonym(s):
2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
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NOK 516.00
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NOK 1,800.00
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NOK 5,610.00
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5 MG
NOK 516.00
25 MG
NOK 1,800.00
100 MG
NOK 5,610.00
About This Item
Linear Formula:
C21H17ClN2O3
CAS Number:
Molecular Weight:
380.82
Beilstein:
6030951
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
Product Name
Rhodamine 123, mitochondrial specific fluorescent dye
Quality Level
form
powder
solubility
ethanol: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
InChI
1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
InChI key
MYFATKRONKHHQL-UHFFFAOYSA-N
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Application
Biochem/physiol Actions
Rhodamine 123 is a fluorescent dye most commonly used in flow cytometry as functional reporter for P-glycoprotein (Pgp).[3] It is part of the rhodamine family of flurone dyes.[4] The dye is mostly used to assess mitochondrial bioenergetics in living cells. Functional assays for multi-drug resistance (MDR) proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of multi-drug resistance protein-1 (MRP1).[4]
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Is Rhodamine 123 suitable for mitochondrial staining?
1 answer-
Helpful?
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Which one is the better concentration for mitochondrial visualization in immunofluoressence microscopy? I´m using mononuclear cells. Thanks.
1 answer-
Testing R8004 for mitochondrial staining is not part of the standard quality control testing for product R8004. Did find a reference that mentions using Rhodamine 123 at 10 ug/ml for the staining of mitochondria
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What are the excitation and emission wavelengths for Rhodamine 123, (Product Nos. 83702 and R8004)?
1 answer-
Using ethanol as the solvent, the excitation maximum is 511 nm and the emission maximum is 534 nm. The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively.
Helpful?
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What is the solubility of Rhodamine 123, products 83702 and R8004?
1 answer-
Rhodamine 123 is soluble in ethanol at 20 mg/mL, and has been reported soluble in ether, various fats and lipids, and has limited solubility in water (no concentration given).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Why would one select Rhodamine 123, products 83702 and R8004, instead of other rhodamine compounds?
1 answer-
Due to is comparatively low toxicity, Rhodamine 123 is the dye of choice for visualizing mitochondria or for cell viability measurements.
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