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MAK208

Sigma-Aldrich

GPDH Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

Glycerol 3-Phosphate Dehydrogenase Activity Assay Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.84

detection method

colorimetric

storage temp.

−20°C

Gene Information

General description

Glycerol 3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) catalyzes the reversible conversion of dihydroxyacetone phosphate and NADH to glycerol-3-phosphate and NAD+.[1][2] It forms a glycerol phosphate shuttle and facilitates the transfer of reduced equivalents across the mitochondria to the cytosol.[3] GPDH is important for both carbohydrate and lipid metabolism.[2] Mutations in GPDH gene result in transient infantile hypertriglyceridemia.[1]

Application

GPDH (glyceraldhyde-3-phoshate dehydrogenase) activity assay kit has been used to measure the serum and hepatic concentrations of GPDH in rabbit.[4]

Suitability

Suitable for the detection of Glycerol-3-Phosphate Dehydrogenase Activity in tissue and cell culture samples.

Principle

The Glycerol 3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit provides a simple and sensitive procedure for measuring GPDH activity in a variety of tissues and cells. GPDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of GPDH is the amount of enzyme required to generate 1.0 μmole of NADH per minute at pH 8 at 37 °C.

Kit Components Only

Product No.
Description

  • GPDH Assay Buffer

  • GPDH Substrate

  • GPDH Probe

  • NADH Standard

  • GPDH Positive Control

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials


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Regulation of adenine nucleotide translocase and glycerol 3-phosphate dehydrogenase expression by thyroid hormones in different rat tissues.
DUMMLER K, et al.
The Biochemical Journal, 317(3), 913-918 (1996)
B. Elmahdi, MA Al-Omair, AA El-Bessoumy and SM El-Bahr.
Thomson I S I
International Journal of Pharmaceutics, 12(4), 429-434 (2016)
A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.
Joshi M, et al.
European Journal of Human Genetics, 22(10), 1229-1229 (2014)
Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 ? resolution.
Alarcon D A, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 68(11), 1279-1283 (2012)

Questions

1–2 of 2 Questions  
  1. What is the substrate used for detection in this kit? The description indicates that it can be used for both GPD1 and GPD2 (mitochondrial GPDH) activity detection.

    1 answer
    1. The identity of the substrate is proprietary and cannot be disclosed. The kit can work for both GDP1 and GDP2.

      Helpful?

  2. Which substrate is used for the detection in the activity assay kit for mGPDH (GPD2)

    1 answer
    1. The substrate used for detection in the MAK208 kit for measuring Glycerol 3-phosphate dehydrogenase (GPDH) activity is dihydroxyacetone. This kit specifically measures cytosolic GPDH, identified by the EC number 1.1.1.8, and it quantifies the production of NADH. Cytosolic GPDH catalyzes the conversion of sn-glycerol 3-phosphate and NAD into NADH, glycerone phosphate, and H+. On the other hand, mitochondrial GPDH, identified by the EC number 1.1.5.3, catalyzes a different reaction involving sn-glycerol 3-phosphate and quinone into glycerone phosphate and quinol.

      Helpful?

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