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MAK082

Sigma-Aldrich

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

ALDH Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cardiovascular diseases; cancer; hematological disorder

storage temp.

−20°C

General description

Aldehyde dehydrogenases (ALDH) are a family of enzymes that catalyze the metabolism of exogenous and endogenous aldehydes, preventing the accumulation of potentially reactive and toxic aldehydes and their metabolites. In addition to their role in aldehyde metabolism, ALDH enzymes also play critical roles in other cellular processes such as cell proliferation, differentiation, and survival.[1][2] Mutations in ALDH family members have been linked to numerous diseases such as heart disease, cataracts, and certain cancers. ALDH activity may also serve as a marker for certain stem cell populations including hematopoietic stem cells and certain cancer stem cells.[3]

Application

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the activity of aldehyde dehydrogenase.[4][5]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of aldehyde dehydrogenase (ALDH) in a variety of samples including tissue and cells

Principle

The Aldehyde Dehydrogenase Activity Colorimetric Assay kit provides a simple and direct procedure for measuring NAD-dependent ALDH activity in a variety of samples. ALDH activity is determined by a coupled enzyme assay in which acetaldehyde is oxidized by ALDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the ALDH activity present. One unit of ALDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H314,H350

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8A24K07310
8G27K07310
7L23K07310
7D06K07310
6K05K07310

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A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.
Yilu Qin et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(24), e2204046-e2204046 (2022-08-26)
Xiaotian Xu et al.
Aging, 14(11), 4858-4873 (2022-06-10)
The roles of asparagine-linked glycosylation (ALG) members in tumorigenic process have been widely explored. However, their effects in colorectal cancer progression are still confusing. Here, we screened 12 ALGs' expression through online datasets and found that ALG10 was mostly upregulated
Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.
Duru N, et al.
Oncotarget, 7(30), 47511-47511 (2016)
Yilu Qin et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8(2), 2002232-2002232 (2021-01-30)
Cancer stem cells (CSCs) are considered the roots of cancer metastasis and recurrence (CSCs), due in part to their self-renewal and therapy resistance properties. However, the underlying mechanisms for the regulation of CSC stemness are poorly understood. Recently, increasing evidence
Ting Liu et al.
Bioengineered, 12(1), 7143-7155 (2021-09-22)
Cancer stem cell (CSC) has been confirmed to trigger tumor occurrence and progression and CSC can develop strategies to maintain a lower reactive oxygen species (ROS) level compared to cancer cells. However, the mechanisms contributing to ROS homeostasis in CSC
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Articles

Cancer Stem Cell Detection with AldeRed™ 588-A

AldeRed™ 588-A detects ALDH activity in cancer stem cells, aiding in cancer research.

Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Is it possible for the assay buffer to lyse the cells without the use of additional instrumentation such as a sonicator?

    1 answer

    1. It is recommended to use a dounce homogenizer on ice or perform mild sonication on ice to lyse the cells. The assay buffer contains detergent but will not lyse the cells by simply incubating in it.

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  4. Is there a general range expected for the activity of the ALDH positive control? Given variances in room temperature, etc.

    1 answer

    1. The positive control does not have an expected activity range.

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  5. What is the kit Sensitivity? thanks,

    1 answer

    1. This assay kit can detect < 0.5 mU/well of ALDH activity (based on the unit definition) in a variety of samples. Unit Definition: One unit is the amount of enzyme that will generate 1.0 umol of NADH per min at pH 8 at room temperature. This is a sensitive assay for measuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses.

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