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MAK064

Sigma-Aldrich

Lactate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Lactic Acid Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

General description

L(+)-Lactate is a metabolic compound formed in animals by the action of the enzyme Lactate Dehydrogenase. Lactate is produced in proliferating cells and during anaerobic conditions such as exercise. Abnormally high concentrations of lactate have been related to pathological conditions such as cancer, diabetes, and lactate acidosis. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. This kit provides a convenient means for detecting L(+)-Lactate in biological samples. There is no need of pretreatment or purification of samples.

Application

Lactate Assay Kit has been used to determine the concentrations of lactate in samples.[1][2]

Suitability

Suitable for detecting L(+)-Lactate in culture medium, fermentation medium, blood and cells.

Principle

In this assay, lactate concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactate present. Typical levels of sensitivity for this kit are between 0.001-10 mM.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8J15K06070
8A17K06070
8B23K06070
8L03K06070
8F30K06070

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Min Xu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1), 151-160 (2018-05-16)
Poor response to chemotherapy leads to the relapse and metastatic progression of tumors. Reprogrammed glucose metabolism is one of the important hallmarks of cancer that facilitates cancer cell survival, proliferation and chemoresistance. However, the precise fate of glucose metabolism and
Ivan Kushkevych et al.
Journal of clinical medicine, 8(7) (2019-07-25)
The small-large intestine axis in hydrogen sulfide accumulation and testing of sulfate and lactate in the gut-gut axis of the intestinal environment has not been well described. Sulfate reducing bacteria (SRB) of the Desulfovibrio genus reduce sulfate to hydrogen sulfide
Cardiac STAT3 Deficiency Impairs Contractility and Metabolic Homeostasis in Hypertension.
Altara R, et al.
Frontiers in Pharmacology, 7, 436-436 (2016)
Annasofia Anemone et al.
International journal of oncology, 51(2), 498-506 (2017-07-18)
Dichloroacetate (DCA) can reverse the glycolytic phenotype that is responsible of increased lactate production and extracellular pH acidification in cancer cells. Magnetic resonance imaging-chemical exchange saturation transfer (MRI-CEST) pH mapping is a novel non-invasive imaging approach that can measure in vivo
Khalid N M Abdelazeem et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(6), 2374-2382 (2017-05-04)
Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H+ ions, which is in part accomplished by
View All Related Papers

Articles

Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

Questions

1–9 of 9 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the formula for the MAK064D Lactate standard?

    1 answer

    1. The standard provided in the MAK064-1KT Lactate Assay Kit is L-Lactic acid sodium salt (CAS 867-56-1) dissolved in water. It contains no other components or cofactors.

      Helpful?

  4. Since the L-(+)-Lactate standard is not a pure solution of sodium L-(+)-Lactate in water or buffer, which are the cofactors present in the standard?

    1 answer

    1. The standard provided in this kit is L-Lactic acid sodium salt (CAS 867-56-1) dissolved in water. There are no other components or cofactors present in the standard.

      Helpful?

  5. What is the concentration range of lactate for this? The description says typical sensitivity is 0.001 - 10 mM, but in the product info the standard curve for colorimetric detection appears to go to a max. concentration of 0.0002µM (10nmol in 50µL sample)

    1 answer

    1. The lower limit of detection is calculated by dividing the lowest concentration standard by the maximum sample volume. The higher limit of detection is determined by dividing the highest concentration standard by the minimum sample volume.

      For the higher limit of detection, take the 10 nmol standard and divide by 1 uL to get 10 mM. For the lower limit of detection, take 0.02 nmol and divide by 50 uL to get 0.0004 mM. We have reached out to our vendor to discuss the range calculation further, as it seems there is some conflicting information.

      Helpful?

  6. Can this kit be used on conditioned media containing phenol red?

    1 answer

    1. If the sample being measured is the cell culture supernatant, the required volume is 20 uL. This is diluted with a total volume of 100 uL of the various kit reagents. The presence of phenol red may impact the results. It would be up to the end user to determine suitability.

      Helpful?

  7. On which enzymatic reaction is this kit based? Does pyruvate in the sample interfere with the assay?

    1 answer

    1. This kit utilizes lactate oxidase to measure lactate. In sufficient amounts pyrivate may impact the results. This can be corrected by subtracting any background from the blank value. See page 3 in the kit protocol provided in the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/411/763/mak064bul.pdf

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Can I make up my own Lactate standard to use with Product MAK064, Lactate Assay kit?

    1 answer

    1. Unfortunately, no. If you run the 100 mM L-(+)-Lactate standard (MAK064D) that comes with the kit, you should get good results. However, if you just use, for example, a solution of sodium L-(+)-lactate dissolved in water or buffer, you probably won't get good results. There are cofactors present in biological samples and in the L-(+)-Lactate standard that comes with the kit (which is not a pure solution of sodium L-(+)-Lactate in water or buffer). If such a "homemade" standard solution were to be spiked with a biological sample or the kit standard, the calculations would be more complicated, but the assay should work.

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