immunohistochemistry (frozen sections): 1:1000 immunoprecipitation (IP): 3 μL using 200 μg of rat cerebellum. indirect immunofluorescence: 1:1000 using frozen sections western blot: 1:1000 using 200 μg cell lysate
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Methods in molecular biology (Clifton, N.J.), 1677, 253-263 (2017-10-08)
Proving endogenous GluN presence and functions in microglia require complementary steps to demonstrate (1) that GluN genes are transcripted and translated, (2) their cellular localization, (3) that the GluN are functional, and (4) the role of the functional GluN. The
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