Our own assay is performed at 30°C. One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives 25°C as another value for the same enzyme from the same species.
L2157
Laccase from Rhus vernicifera
crude acetone powder, ≥50 units/mg solid
Synonym(s):
Benzenediol:oxygen oxidoreductase
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10000 UNITS
NOK 1,770.00
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10000 UNITS
NOK 1,770.00
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
form
crude acetone powder
Quality Level
specific activity
≥50 units/mg solid
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
storage temp.
−20°C
SMILES string
[n]1(c(c(cc1C)C=O)C)CC
InChI
1S/C9H13NO/c1-4-10-7(2)5-9(6-11)8(10)3/h5-6H,4H2,1-3H3
InChI key
NWDZDFOKSUDVJV-UHFFFAOYSA-N
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell and cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Unit Definition
One unit will produce a ΔA530 of 0.001 per min at pH 6.5 at 30 °C in a 3 mL reaction volume using syringaldazine as substrate.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Laccase, a biocatalyst, oxidizes aromatic compounds with molecular oxygen reduction, making it useful in various applications.
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What is the temperature optimum of Product L2157, Laccase from Rhus vernicifera?
1 answer-
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My solution of Product L2157, Laccase from Rhus vernicifera, isn't clear. Is this normal?
1 answer-
Yes. L2157 is a crude acetone powder, and can contain other proteins, enzymes, and cellular debris. A suspension may be prepared in 100 mM potassium phosphate buffer, pH 6.5, by homogenization with a hand-held homogenizer for 10 minutes. This preparation may then be centrifuged; the laccase activity will be present in the supernatant.
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What is the pH optimum of Product L2157, Laccase from Rhus vernicifera?
1 answer-
Our own assay is performed at pH 6.5. One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives pH 6.5 as another value for the laccase from this species, with a range for enzyme activity of 4.3 to 9.
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How stable are solutions of Product L2157, Laccase from Rhus vernicifera?
1 answer-
Solutions should be prepared fresh, directly before use, as they are not stable as stock solutions.
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What is the molecular weight (MW) of Product L2157, Laccase from Rhus vernicifera?
1 answer-
Spira-Solomon and Solomon, J. Am. Chem. Soc., 109, 6421-6432 (1987) reports estimated molecular weights of 52 kDa (laccase 1), 55 kDa (laccase 2), and 70 kDa (laccase 3), as determined by gel filtration.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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