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H7411
Anti-HA−FITC antibody, Mouse monoclonal
clone HA-7, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
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100 μG
NOK 6,260.00
NOK 6,260.00
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100 μG
NOK 6,260.00
About This Item
NOK 6,260.00
Please contact Customer Service for Availability
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biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous solution
storage condition
protect from light
concentration
1 mg/mL
technique(s)
immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde.
shipped in
wet ice
storage temp.
−20°C
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General description
Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.
Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.[1]
Specificity
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Application
Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:
- in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells[2]
- in western blotting detection of spindle-associated proteins in Trypanosoma brucei[3]
- in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies[4]
Biochem/physiol Actions
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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What is the Department of Transportation shipping information for this product?
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What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
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Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
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