D7385

Demecolcine

≥98% (HPLC)

• Synonym(s): N-Deacetyl-N-methylcolchicine
• Empirical Formula (Hill Notation): C₂₁H₂₅NO₅
• CAS Number: 477-30-5
• Molecular Weight: 371.43
• Beilstein: 2822892
• EC Number: 207-514-6
• MDL number: MFCD00075459
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894128
• NACRES: NA.47

Properties

• Quality Level: 200
• Assay: ≥98% (HPLC)
• form: powder
• mp: 184 °C
• solubility: chloroform: 10 mg/mL; ethanol: soluble
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
• InChI key: NNJPGOLRFBJNIW-HNNXBMFYSA-N

Description

Application

Demecolcine has been used as a microtubule depolymerizing agent. It has also been used to inhibit mitotic division.

Biochem/physiol Actions

Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G₁ and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H300
• Precautionary Statements: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves