Ri-1

96090512, human blood, Lymphoblastoid

• Synonym(s): Ri-1 Cell Line
• UNSPSC Code: 41106514

Properties

• product name: Ri-1, 96090512
• biological source: human blood
• growth mode: Suspension
• karyotype: 92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian B-cell lymphoma

Cell Line Description

The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.

Application

Study of B-cell lymphoma-leukaemia

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.

Other Notes

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