EMT6/VRP/R
96042319, mouse unknown/unspecified, Epithelial-like
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About This Item
UNSPSC Code:
41106514
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product name
EMT6/VRP/R, 96042319
biological source
mouse unknown/unspecified
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse mammary, drug-resistant
Cell Line Description
The drug-resistant line EMT6/VRP/R was established from the parent cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in verapamil (see reference).
Application
Drug-resistance studies
Culture Medium
MEM (EBSS) +2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 75 μg/ml verpamil + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37ºC. It is recommended to resuscitate cells in drug-free medium. Add drug after growth is established.
Other Notes
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Certificates of Analysis (COA)
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