SNU-C2B

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human intestine
• packaging: tube of 5 μg 96020932-DNA-5UG; pkg of vial of cells 96020932-1VL
• growth mode: Semi-adherent aggregates
• karyotype: Pseudodiploid. Modal chromosome number is 46 at 70% with polyploidy at 16.4%. The del(3) (p23), t(4,?), t(8;?) (p22), del (9) (p13), del (11) (p13), t(1;8) (p13;p24), t(1;?) (q11,?) and 2 other marker chromosomes, if any, occur only sporadically.
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Colorectal Carcinoma

Cell Line Description

This cell line was derived from a fourth passage nude mouse xenograft of tumour fragments obtained from a 43 year old Asian female patient prior to therapy. The cells have a high concentration of receptors for EGF. This cell line has lost both chromosomes 8 and 11. This is one of 14 colorectal carcinoma cell lines derived by JG Park and associates during the years 1982 to 1985. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.0mM sodium pyruvate

Subculture Routine

Split sub-confluent cultures (70-80 %) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37 C; 5% CO₂.

Other Notes

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