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Key Documents

F10-150.58

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-LFA-1, sheep, 93100509

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Mouse Hybridoma

packaging

tube of 5 μg 93100509-DNA-5UG
pkg of vial of cells 93100509-1VL

ImmunoDon

BALB/c mouse spleen

Myeloma

Sp2/0

Reactivity

Anti-LFA-1, sheep

technique(s)

cell culture | mammalian: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse Sp2/0 x Mouse BALB/c spleen

Cell Line Description

The antibody recognises the sheep LFA-1 molecule expressed by virtually all leukocytes. There is species cross-reactivity with goat.

Immunogen

Sheep lymphocytes

Application

FACS

Culture Medium

IDMEM + 2mM glutamine + 1% Hypoxanthine, Aminopterin, Thymidine (HAT) + 10% Foetal Bovine Serum (FBS) (may benefit from IL-6 in medium)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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