HeLa9903
11033105, human cervix, Epithelial
Synonym(s):
HeLa
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About This Item
UNSPSC Code:
41106514
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Product Name
HeLa9903, 11033105, from human cervix
biological source
human cervix
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
HeLa, genetic modification, the hER alpha expression construct, Oestrogen-Responsive Element
Cell Line Description
HeLa9903 is a HeLa cell line stably transfected with two constructs: the hER alpha expression construct; a firefly luciferase reporter construct bearing Oestrogen-Responsive Element (ERE) driven by a mouse metallothionein (MT) promoter TATA element. HeLa9903 can be used to test for evaluation of chemicals based on OECD Test Guideline: The stably transformed human oestrogen receptor-alpha transcriptional; activation assay for detection of oestrogenic activity of chemicals.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Culture Medium
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 16μg/ml Blasticidin S HCL + 800μg/ml G418.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Legal Information
US7537904B1 N Ooe & Matsunaga H 2009.Cell for measuring the ability to control the activity of a ligand-responsive transcription control factor.
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