B9304
Anti-Bcl-x antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-BCL2L1
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About This Item
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 26-30 kDa
species reactivity
mouse, human, rat
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human colon adenocarcinoma sections
microarray: suitable
western blot: 1:2,000 using human Burkit lymphoma Raji cell lysate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BCL2L1(598)
mouse ... Bcl2l1(12048)
rat ... Bcl2l1(24888)
General description
Bcl-2-like protein 1 (BCL2L1) or Bcl-2-related gene x, long isoform (Bcl-x) gene is mapped to human chromosome 20q11.21. BCL2L1 belongs to the Bcl-2 family. The bcl-x gene on alternative splicing leads two distinct proteins. It comprises multiple Bcl-2 homology (BH3) domains. Bcl-xL is expressed in the adult brain.(10)
Specificity
The antibody reacts with Bcl-xL.
Immunogen
synthetic peptide corresponding to the N-terminal of human Bcl-x (amino acids 2-20) conjugated to BSA. This sequence is identical in human and mouse Bcl-xS, Bcl-xL, Bcl-Xβ and Bcl-Xγ isoforms and is highly conserved in rat Bcl-xS and Bcl-xL (single amino acid substitution).
Application
Biochem/physiol Actions
Bcl-2-like protein 1 (BCL2L1) or Bcl-2-related gene x arelong isoforms (Bcl-x) of proteins that are involved in regulating apoptosis. Similar to Bcl-2, Bcl-xL can inhibit cell death upon growth factor removal, when transfected in an IL-3 dependent cell line. Bcl-xL can form ion channels in biological membranes and may maintain cell survival by regulating the permeability of intracellular membranes. Bcl-xL may bind to apoptotic protease activating factor 1 (Apaf-1) preventing it from activating caspase 9. Like Bcl-2, Bcl-x activity is regulated by specific phosphorylation of a 60-amino acid regulatory loop region. Bcl-xL has been shown to regulate cell response to oxidative stress, cellular resistance to DNA damage-induced apoptosis by chemotherapeutic agents and ionizing radiation, and favors neurite outgrowth.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month.For extended storage, freeze in working aliquots.Repeated freezing and thawing is not recommended.Storage in "frost-free" freezers is not recommended. Ifslight turbidity occurs upon prolonged storage, clarifythe solution by centrifugation before use. Workingdilution samples should be discarded if not used within12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Marisa A Cubilla et al.
Investigative ophthalmology & visual science, 54(1), 313-322 (2012-12-06)
Glucocorticoids are best known by their protective effect on retinal photoreceptor damage. However, they could also be involved in photoreceptor homeostasis under basal, nonstressful conditions. Therefore, we aimed to study glucocorticoid-induced changes of survival-related molecules in male mice retinas under
Jianfang Wang et al.
Cellular signalling, 23(12), 2030-2038 (2011-08-16)
Functional analysis of a Bcl-xL phosphorylation mutant series has revealed that cells expressing Bcl-xL(Ser49Ala) mutant are less stable at G2 checkpoint after DNA damage and enter cytokinesis more slowly after microtubule poisoning, than cells expressing wild-type Bcl-xL. These effects of
Ayumi Nakamura et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(20), 5448-5461 (2016-05-20)
Apoptosis plays an essential role during brain development, yet the precise mechanism by which this pathway is regulated in the brain remains unknown. In particular, mammalian cells are known to express multiple anti-apoptotic Bcl-2 family proteins. However, the cells of
A J Minn et al.
Nature, 385(6614), 353-357 (1997-01-23)
Bcl-2-related proteins are critical regulators of cell survival that are localized to the outer mitochondrial, outer nuclear and endoplasmic reticulum membranes. Despite their physiological importance, the biochemical function of Bcl-2-related proteins has remained elusive. The three-dimensional structure of Bcl-xL, an
Megan Stevens et al.
Frontiers in genetics, 10, 804-804 (2019-09-26)
Apoptosis plays a vital role in cell homeostasis during development and disease. Bcl-x, a member of the Bcl-2 family of proteins, is a mitochondrial transmembrane protein that functions to regulate the intrinsic apoptosis pathway. An alternative splicing (AS) event in
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