Skip to Content
Merck
All Photos(1)

Documents

B7405

Sigma-Aldrich

Anti-Digoxin−Biotin antibody, Mouse monoclonal

clone DI-22, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin−Biotin antibody produced in mouse

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DI-22, monoclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:10,000 using digoxin-labeled biomolecule
western blot: 1:500 using using digoxin-labeled biomolecule

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Immunogen

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse has been used in:
  • in situ hybridization
  • immunohistochemistry
  • immunolocalization

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse may be used for ELISA at a dilution of 1:10,000 and for dot blots at a dilution of 1:500. It was also used to stain apoptotic cells in cervical carcinoma sections by TUNEL technique.

Biochem/physiol Actions

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
The conjugate may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Anti-Digoxigenin-AP, Fab fragments from sheep

Roche

11093274910

Anti-Digoxigenin-AP, Fab fragments

Il Soo Moon et al.
Journal of Korean medical science, 27(10), 1241-1247 (2012-10-24)
Long-term synaptic plasticity requires addition of new proteins at the synaptic site. The local protein synthesis at subsynaptic sites confers advantageous mechanisms that would regulate the protein composition in local domains on a moment-by-moment basis. However, our information on the
Mary C Bedard et al.
Cancers, 13(11) (2021-06-03)
Juvenile-onset recurrent respiratory papillomatosis (JoRRP) is the most common benign neoplasm of the larynx in children, presenting with significant variation in clinical course and potential for progression to malignancy. Since JoRRP is driven by human papillomavirus (HPV), we evaluated viral
RIPK1 protects from TNF-alpha-mediated liver damage during hepatitis
Filliol A, et al.
Cell Death & Disease, 7(11), e2462-e2462 (2016)
Differences in apoptotic status in the bovine placentome between spontaneous and induced parturition
HIrAyAmA H, et al.
Journal of Reproduction and Development (2012)
F D Cuello-Carrión et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 47(6), 837-839 (1999-05-20)
We are in the process of assessing the response of cancer tissues to chemotherapy, evaluating, among other points, the proportion of cancer cells undergoing apoptosis. However, the apoptotic index obtained with the original TUNEL technique was lower than that obtained
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service