A0847
Adenylyl Cyclase Toxin from Bordetella pertussis
≥70% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, ≥50 units/mg protein
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About This Item
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recombinant
expressed in E. coli
Quality Level
Assay
≥70% (SDS-PAGE)
form
lyophilized powder
specific activity
≥50 units/mg protein
mol wt
177 kDa by amino acid sequence
storage temp.
2-8°C
Gene Information
human ... CAMP(820)
mouse ... CAMP(12796)
rat ... CAMP(316010)
Biochem/physiol Actions
Adenylate cyclase toxin from Bordetella pertussis belongs to the family of pore-forming toxins. It converts host ATP to cyclic AMP (cAMP). May be used to affect intracellular cAMP levels in mammalian cells.
Unit Definition
One unit will produce 1.0 μmol cAMP from ATP per minute at pH 8.0 at 30 °C
Reconstitution
When reconstituted with 50 μL of water, the protein is in 0.05 M Tris, 8 M urea, 0.002 M EDTA, pH 8.0.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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