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Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside

≥98.0% (HPLC), powder or crystals

Synonym(s):

X-α-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
107021-38-5
Molecular Weight:
408.63
MDL number:
MFCD00063780
UNSPSC Code:
12352204
PubChem Substance ID:
329751267
NACRES:
NA.32

product name

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside, ≥98.0% (HPLC)

Assay

≥98.0% (HPLC)

form

powder or crystals

optical activity

[α]/D 135.0±5.0°, c = 1 in DMF/H2O 1:1

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14+/m1/s1

InChI key

OPIFSICVWOWJMJ-YGEXGZRRSA-N

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Application

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside is suitable as a substrate for α-galactosidase. It has been used:
  • in the yeast two-hybrid screen
  • for differentiating α-galactosidase-positive strains of yeast
  • to determine melibiase activity in yeast and to identify Saccharomyces cerevisiae A241 × Saccharomyces uvarum C995 hybrids

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

related product

Product No.
Description
Pricing

94433

β-Galactosidase stain

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tubb, R.S. and Liljestrom, P.L.
Journal of the Institute of Brewing, 92, 588-588 (1986)
A novel reporter gene MEL1 for the yeast two-hybrid system.
S Aho et al.
Analytical biochemistry, 253(2), 270-272 (1997-12-31)
A Unique Saccharomyces cerevisiae ? Saccharomyces uvarum Hybrid Isolated From Norwegian Farmhouse Beer: Characterization and Reconstruction
Krogerus K, et al.
Frontiers in Microbiology, 9 (2018)
Jiaojie Li et al.
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The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies to produce and spin recombinant fibrous proteins are necessary. This protocol describes silk

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